let-7 microRNA

Sequences

• Seed sequences
• Seed alignment
• Known sequences used for evaluating
• Erpin epn file, Erpin parameters: -4,9 -add 4 -pcw 101 -cutoff 34.64

Descriptor 1st iteration

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parms
wc += gu;
descr
h5 (tag='H1', minlen=3, maxlen=6)
ss (tag='S1', minlen=1, maxlen=2)
h5 (tag='H2', minlen=21, maxlen=21, mispair=2, seq="^GAGGUAG")
ss (tag='S2', minlen=0, maxlen=4)
h5 (tag='H3', minlen=2, maxlen=4, mispair=1)
ss (tag='S3', minlen=3, maxlen=12)
h3 (tag='H3')
ss (tag='S4', minlen=7, maxlen=13)
h3 (tag='H2')
ss (tag='S5', minlen=0, maxlen=2)
h3 (tag='H1')

Descriptor 2nd iteration

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• Diff to desc1
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parms
wc += gu;
descr
h5 (tag='H1', minlen=3, maxlen=6)
ss (tag='S1', minlen=1, maxlen=2)
h5 (tag='H2', minlen=21, maxlen=21, mispair=2, seq="^GAGGUAGNANNUUGNNNNGUU")
ss (tag='S2', minlen=0, maxlen=4)
h5 (tag='H3', minlen=2, maxlen=4, mispair=1)
ss (tag='S3', minlen=3, maxlen=12)
h3 (tag='H3')
ss (tag='S4', minlen=7, maxlen=13)
h3 (tag='H2')
ss (tag='S5', minlen=0, maxlen=2)
h3 (tag='H1')

Descriptor 3rd iteration

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• Diff to desc2
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parms
wc += gu;
descr
h5 (tag='H1', minlen=3, maxlen=6)
ss (tag='S1', minlen=0, maxlen=2)
h5 (tag='H2', minlen=19, maxlen=23, mispair=2, seq="^GAGGUAGNANNUUGNNNNGUU", mismatch=2)
ss (tag='S2', minlen=0, maxlen=4)
h5 (tag='H3', minlen=2, maxlen=4, mispair=1)
ss (tag='S3', minlen=3, maxlen=12)
h3 (tag='H3')
ss (tag='S4', minlen=7, maxlen=13)
h3 (tag='H2')
ss (tag='S5', minlen=0, maxlen=2)
h3 (tag='H1')

Result table

The numbers in the "Rank" columns denote the rank of the hit(s), that match the annotated sequence(s). If the Rank column is empty, none of the annotated sequences was among the hits. The E-values are the range from the highest to the lowest rank.