Publications - Published papers

Please find below publications of our group. Currently, we list 500 papers. Some of the publications are in collaboration with the group of Sonja Prohaska and are also listed in the publication list for her individual group. Access to published papers (access) is restricted to our local network and chosen collaborators. If you have problems accessing electronic information, please let us know:

©NOTICE: All papers are copyrighted by the authors; If you would like to use all or a portion of any paper, please contact the author.

MINCR is a MYC-induced lncRNA able to modulate MYC's transcriptional network in Burkitt lymphoma cells.

Doose, Gero and Haake, Andrea and Bernhart, Stephan H. and L\'opez, Cristina and Duggimpudi, Sujitha and Wojciech, Franziska and Bergmann, Anke K. and Borkhardt, Arndt and Burkhardt, Birgit and Claviez, Alexander and Dimitrova, Lora and Haas, Siegfried and Hoell, Jessica I. and Hummel, Michael and Karsch, Dennis and Klapper, Wolfram and Kleo, Karsten and Kretzmer, Helene and Kreuz, Markus and Küppers, Ralf and Lawerenz, Chris and Lenze, Dido and Loeffler, Markus and Mantovani-Löffler, Luisa and Möller, Peter and Ott, German and Richter, Julia and Rohde, Marius and Rosenstiel, Philip and Rosenwald, Andreas and Schilhabel, Markus and Schneider, Markus and Scholz, Ingrid and Stilgenbauer, Stephan and Stunnenberg, Hendrik G. and Szczepanowski, Monika and Trümper, Lorenz and Weniger, Marc A. and ICGC MMML-Seq Consortium, and Hoffmann, Steve and Siebert, Reiner and Iaccarino, Ingram

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Proceedings of the National Academy of Sciences of the United States of America 112 (38): E5261--E5270

Abstract


Despite the established role of the transcription factor MYC in cancer, little is known about the impact of a new class of transcriptional regulators, the long noncoding RNAs (lncRNAs), on MYC ability to influence the cellular transcriptome. Here, we have intersected RNA-sequencing data from two MYC-inducible cell lines and a cohort of 91 B-cell lymphomas with or without genetic variants resulting in MYC overexpression. We identified 13 lncRNAs differentially expressed in IG-MYC-positive Burkitt lymphoma and regulated in the same direction by MYC in the model cell lines. Among them, we focused on a lncRNA that we named MYC-induced long noncoding RNA (MINCR), showing a strong correlation with MYC expression in MYC-positive lymphomas. To understand its cellular role, we performed RNAi and found that MINCR knockdown is associated with an impairment in cell cycle progression. Differential gene expression analysis after RNAi showed a significant enrichment of cell cycle genes among the genes down-regulated after MINCR knockdown. Interestingly, these genes are enriched in MYC binding sites in their promoters, suggesting that MINCR acts as a modulator of the MYC transcriptional program. Accordingly, MINCR knockdown was associated with a reduction in MYC binding to the promoters of selected cell cycle genes. Finally, we show that down-regulation of Aurora kinases A and B and chromatin licensing and DNA replication factor 1 may explain the reduction in cellular proliferation observed on MINCR knockdown. We, therefore, suggest that MINCR is a newly identified player in the MYC transcriptional network able to control the expression of cell cycle genes.