Publications - Published papers

Please find below publications of our group. Currently, we list 500 papers. Some of the publications are in collaboration with the group of Sonja Prohaska and are also listed in the publication list for her individual group. Access to published papers (access) is restricted to our local network and chosen collaborators. If you have problems accessing electronic information, please let us know:

©NOTICE: All papers are copyrighted by the authors; If you would like to use all or a portion of any paper, please contact the author.

Alterations of microRNA and microRNA-regulated messenger RNA expression in germinal center B-cell lymphomas determined by integrative sequencing analysis.

Hezaveh, Kebria and Kloetgen, Andreas and Bernhart, Stephan H. and Mahapatra, Kunal Das and Lenze, Dido and Richter, Julia and Haake, Andrea and Bergmann, Anke K. and Brors, Benedikt and Burkhardt, Birgit and Claviez, Alexander and Drexler, Hans G. and Eils, Roland and Haas, Siegfried and Hoffmann, Steve and Karsch, Dennis and Klapper, Wolfram and Kleinheinz, Kortine and Korbel, Jan and Kretzmer, Helene and Kreuz, Markus and Küppers, Ralf and Lawerenz, Chris and Leich, Ellen and Loeffler, Markus and Mantovani-Loeffler, Luisa and L\'opez, Cristina and McHardy, Alice C. and Möller, Peter and Rohde, Marius and Rosenstiel, Philip and Rosenwald, Andreas and Schilhabel, Markus and Schlesner, Matthias and Scholz, Ingrid and Stadler, Peter F. and Stilgenbauer, Stephan and Sungalee, Stéphanie and Szczepanowski, Monika and Trümper, Lorenz and Weniger, Marc A. and Siebert, Reiner and Borkhardt, Arndt and Hummel, Michael and Hoell, Jessica I. and ICGC MMML-Seq Project,

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PREPRINT 18-029:

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Haematologica 101 (11): 1380--1389

Abstract


MicroRNA are well-established players in post-transcriptional gene regulation. However, information on the effects of microRNA deregulation mainly relies on bioinformatic prediction of potential targets, whereas proof of the direct physical microRNA/target messenger RNA interaction is mostly lacking. Within the International Cancer Genome Consortium Project ``Determining Molecular Mechanisms in Malignant Lymphoma by Sequencing'', we performed miRnome sequencing from 16 Burkitt lymphomas, 19 diffuse large B-cell lymphomas, and 21 follicular lymphomas. Twenty-two miRNA separated Burkitt lymphomas from diffuse large B-cell lymphomas/follicular lymphomas, of which 13 have shown regulation by MYC. Moreover, we found expression of three hitherto unreported microRNA. Additionally, we detected recurrent mutations of hsa-miR-142 in diffuse large B-cell lymphomas and follicular lymphomas, and editing of the hsa-miR-376 cluster, providing evidence for microRNA editing in lymphomagenesis. To interrogate the direct physical interactions of microRNA with messenger RNA, we performed Argonaute-2 photoactivatable ribonucleoside-enhanced cross-linking and immunoprecipitation experiments. MicroRNA directly targeted 208 messsenger RNA in the Burkitt lymphomas and 328 messenger RNA in the non-Burkitt lymphoma models. This integrative analysis discovered several regulatory pathways of relevance in lymphomagenesis including Ras, PI3K-Akt and MAPK signaling pathways, also recurrently deregulated in lymphomas by mutations. Our dataset reveals that messenger RNA deregulation through microRNA is a highly relevant mechanism in lymphomagenesis.