Publications - Published papers

Please find below publications of our group. Currently, we list 500 papers. Some of the publications are in collaboration with the group of Sonja Prohaska and are also listed in the publication list for her individual group. Access to published papers (access) is restricted to our local network and chosen collaborators. If you have problems accessing electronic information, please let us know:

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Polo-like kinase 4 transcription is activated via CRE and NRF1 elements, repressed by DREAM through CDE/CHR sites and deregulated by HPV E7 protein

Martin Fischer, Marianne Quaas, Axel Wintsche, Gerd A. Müller and Kurt Engeland

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Nucleic Acids Research, 2013, 1–18

Abstract


Infection by oncogenic viruses is a frequent cause for tumor formation as observed in cervical cancer. Viral oncoproteins cause inactivation of p53 function and false transcriptional regulation of central cell cycle genes. Here we analyze the regulation of <em>Plk4</em>, serving as an example of many cell cycle- and p53-regulated genes. Cell cycle genes are often repressed via CDE and CHR elements in their promoters and activated by NF-Y binding to CCAAT-boxes. In contrast, general activation of <em>Plk4</em> depends on NRF1 and CRE sites. Bioinformatic analyses imply that NRF1 and CRE are central elements of the transcriptional network controlling cell cycle genes. We identify CDE and CHR sites in the <em>Plk4</em> promoter, which are necessary for binding of the DREAM (<u>D</u>P, <u>R</u>B-like, <u>E</u>2F4 <u">a</u>nd <u>M</u>uvB) complex and for mediating repression in G<sub>0</sub>/G<sub>1</sub>. When cells progress to G<sub>2</sub> and mitosis, DREAM is replaced by the MMB (<span class="underline">M</span>yb-<span class="underline">M</span>uv<span class="underline">B</span>) complex that only requires the CHR element for binding. <em>Plk4</em> expression is downregulated by the p53-p21<sup>WAF1/CIP1</sup>-DREAM signaling pathway through the CDE and CHR sites. Cell cycle- and p53-dependent repression is abrogated by HPV E7 oncoprotein. Together with genome-wide analyses our results imply that many cell cycle genes upregulated in tumors by viral infection are bound by DREAM through CDE/CHR sites.