Publications - Published papers

Please find below publications of our group. Currently, we list 501 papers. Some of the publications are in collaboration with the group of Sonja Prohaska and are also listed in the publication list for her individual group. Access to published papers (access) is restricted to our local network and chosen collaborators. If you have problems accessing electronic information, please let us know:

©NOTICE: All papers are copyrighted by the authors; If you would like to use all or a portion of any paper, please contact the author.

Size Variation and Structural Conservation of Vertebrate Telomerase RNA

Mingyi Xie, Axel Mosig, Xiaodong Qi, Yang Li, Peter F. Stadler, Julian J.-L. Chen

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J. Chem. Biol. 283: 2049-2059 (2008)

Abstract


Telomerase extends chromosome ends by copying a short template sequence within its intrinsic RNA component. Telomerase RNA (TR) from different groups of species varies dramatically in sequence and size. We report here the bioinformatic identification, secondary structure comparison and functional analysis of the smallest known vertebrate TRs from five teleost fishes. The teleost TRs (312-348 nt) are significantly smaller than the large cartilaginous fish TRs (478-559 nt) and tetrapod TRs. This remarkable length reduction of teleost fish TRs correlates positively with their genome size, reflecting an unusual structural plasticity of TR during genome evolution. The teleost TR structure, lacking most of the variable sequences, defines the minimum consensus of the vertebrate TR structure. Despite their small sizes, the medaka and fugu TRs, when assembled with their catalytic reverse transcriptase protein counterparts, reconstituted active and processive enzymes. The CR4-CR5 domain of teleost fish TR is relatively small and yet displays a higher efficiency than the pseudoknot domain in reconstituting telomerase activity. With the identification and characterization of teleost fish TR, we now have a complete view of the evolutionary divergence of vertebrate TR.

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doi/10.1074/jbc.M708032200