Syntheny analysis of Monosiga brevicollis (MonBre) and Salpingoeca rosetta (SalRos) CCA gene loci. Twenty flanking genes upstream and downstream of the focal CCA genes were taken from gene annotations. Gene products of these 160 genes were run against the NCBI protein database. From each run, the top blast hit was kept if it exceeded the threshold (e-value lower than 1.0e-5) and classified according to taxonomic groups (x axis). NF -- not found. This shows that most genes flanking the CCA genes have their closest relatives among Deuterostomia, however, only one of 160 sequences is most similar to human.